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Journal / Immunology and Infection / Schnelle Analyse von Chromosomenaberrationen in…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Schnelle Analyse von Chromosomenaberrationen in Maus-B-Lymphozyten durch PNA-FISH
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Schnelle Analyse von Chromosomenaberrationen in Maus-B-Lymphozyten durch PNA-FISH

Article DOI: 10.3791/51806-v • 07:54 min • August 19th, 2014
August 19th, 2014 •
1, 1
1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey

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DNA-Reparaturwege sind wichtig für die Erhaltung der genomischen Integrität und Mutation verhindert und Krebs. Das Ziel des Protokolls ist es, genomische Instabilität durch direkte Beobachtung von Chromosomenaberrationen in Metaphase-Spreads Quantifizierung von Maus-B-Zellen unter Verwendung von Fluoreszenz-in situ-Hybridisierung (FISH) für Telomer-DNA-Wiederholungen.

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Immunologie genomische Instabilität DNA-Reparatur,-Maus Metaphasespreitung Fisch Primärkultur
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