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Journal / Immunology and Infection / Snelle analyse van chromosoomafwijkingen in Muis…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Snelle analyse van chromosoomafwijkingen in Muis B-lymfocyten door PNA-FISH
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Snelle analyse van chromosoomafwijkingen in Muis B-lymfocyten door PNA-FISH

Article DOI: 10.3791/51806-v • 07:54 min • August 19th, 2014
August 19th, 2014 •
1, 1
1Department of Molecular Biology and Biochemistry, Rutgers, the State University of New Jersey

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DNA herstelmechanismen zijn essentieel om de genomische integriteit en preventie mutatie en kanker. Het doel van dit protocol is om instabiliteit van het genoom te kwantificeren door middel van directe observatie van chromosoomafwijkingen in metafase verspreidt van de muis B-cellen met behulp van fluorescente in situ hybridisatie (FISH) voor telomeer DNA herhalingen.

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Immunologie instabiliteit van het genoom DNA reparatie muis metafase spread FISH primaire cultuur
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