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Cytosolisk Kalsium Målinger i nyre Epitelceller med flowcytometri
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Cytosolisk Kalsium Målinger i nyre Epitelceller med flowcytometri

Article DOI: 10.3791/51857-v • 10:24 min • October 28th, 2014
October 28th, 2014 •
1, 2
1Institute for Physiology, Pathophysiology, & Toxicology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke, 2Institute for Immunology & Experimental Oncology, Centre for Biomedical Research and Training (ZBAF), University of Witten/Herdecke

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Kalsium er involvert i en rekke fysiologiske og patofysiologiske signalveier. Levende celle bildebehandling krever spesialutstyr og kan være tidkrevende. En rask, enkel fremgangsmåte ved å bruke et strømningscytometer for å bestemme relative endringer i cytosolisk kalsium i adherente epitelceller bringes i suspensjon ble optimalisert.

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Cellular Biology Nyre FACS andre messenger proksimale tubuli kalsium indikatorer probenecid endoplasmatisk retikulum ionomycin
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