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Bioengineering

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JoVE Journal Bioengineering
Bestilling enkelte celler og Single Embryoner i 3D Indespærring
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Bestilling enkelte celler og Single Embryoner i 3D Indespærring: En ny enhed for High Content Screening

Article DOI: 10.3791/51880-v • 14:22 min • September 18th, 2016
September 18th, 2016 •
1,2, 1,2, 1,2, 1,2
1Laboratory of Cell Physics, Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg, 2Development and Stem Cells Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS and Université de Strasbourg

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Vi rapporterer en indretning og en ny metode til at studere celler og embryoner. Enkelte celler er præcist bestilles i microcavity arrays. Deres 3D indespærring er et skridt i retning 3D-miljøer er stødt på i fysiologiske forhold og tillader organel orientering. Ved at styre celleform, denne opsætning minimerer variation rapporteret i standard assays.

Tags

Bioengineering mikrokaviteter Replica Molding mikrofabrikation High-indhold screening 3-dimensioner enkelte celler Nucleus Golgi Cytokinetic Ring.
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