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Immunodetektion af ydre membranproteiner ved flowcytometri Isoleret Mitokondrier
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Immunodetektion af ydre membranproteiner ved flowcytometri Isoleret Mitokondrier

Article DOI: 10.3791/51887-v • 11:53 min • September 18th, 2014
September 18th, 2014 •
1, 2, 2
1Department of Biochemistry, Université de Montréal, CRCHUM, 2Department of Neurosciences, Université de Montréal, CRCHUM

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Beskrevet her er en metode til at påvise og kvantificere mitokondriske ydre membranproteiner ved immunolabeling mitokondrier isoleret fra gnaver væv og analyse ved flowcytometri. Denne fremgangsmåde kan udvides til at vurdere funktionelle aspekter af mitokondrielle subpopulationer.

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Cellular Biology mitokondrier flowcytometri organel isolation immunmærkning rygmarv TMRM
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