JoVE Journal
Bioengineering
Bioengineering
A subscription to JoVE is required to view this content.
Chapters
Summary
We describe here an improved Luminescence Resonance Energy Transfer (LRET) method where we introduce a protease cleavage site between the donor and acceptor fluorophore sites. This modification allows us to obtain specific LRET signals arising from membrane proteins of interest, allowing for the study of membrane proteins without protein purification.