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JoVE Journal Biology
Generatie
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
Journal (Biology)
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
Journal (Biology)
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
 
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Generatie

Article DOI: 10.3791/51934-v • 13:31 min • October 31st, 2014
October 31st, 2014 •
1
1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University

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Enterobacter sp. YSU groeit in glucose minimale zouten medium. Auxotrofen worden gegenereerd door transformatie met een transposome die willekeurig plaatst zichzelf in het gastheergenoom. Mutanten worden gevonden door replica plating van complexe medium tot minimaal medium. Onderbroken genen worden geïdentificeerd door gen-rescue en sequencing.

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Microbiologie auxotroph transposome transposon mutagenese replica plating glucose minimaal medium complex medium,
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