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JoVE Journal Biology
Generering av
The Establishment of a Murine Mandibular Molar Extraction Socket Healing Model
Journal (Biology)
The Establishment of a Murine Mandibular Molar Extraction Socket Healing Model
Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
Journal (Biology)
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Generering av

Article DOI: 10.3791/51934-v • 13:31 min • October 31st, 2014
October 31st, 2014 •
1
1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University

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Enterobacter sp. YSU växer i glukosminimalt saltmedium. Auxotrofer genereras genom att transformera den med en transposome som slumpvis infogar sig själv in i värdgenomet. Mutanter hittas av replica plating från komplext medium till minimalmedium. Avbrutna gener identifieras genom gen räddning och sekvensering.

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Mikrobiologi auxotrof transposome transposon mutagenes replica plating glukosminimalt medium komplext medium,
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