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Üretilmesi
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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Üretilmesi

Article DOI: 10.3791/51934-v • 13:31 min • October 31st, 2014
October 31st, 2014 •
1
1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University

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Enterobacter sp. YSU glukoz az tuz ortamında büyür. Oksotroflan rastgele bir konakçı genom içine kendisini ekleyen bir transposome ile dönüştürülmesiyle oluşturulur. Mutantlar minimal orta karmaşık ortamdan replika plaka tarafından bulundu. Kesilen gen, kurtarma ve dizileme ile tespit edilir.

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Mikrobiyoloji Sayı 92 auxotroph transposome transposon mutajenez çoğaltma kaplama glikoz minimal ortam karmaşık orta,
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