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JoVE Journal
Immunology and Infection

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传播

Article DOI: 10.3791/51953-v 12:52 min September 25th, 2014
September 25th, 2014

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Summary

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在这里,我们提出了一个协议来传播Homalodisca vitripennis细胞和HoCV-1 体外 。培养基从HoCV-1阳性培养去除和RNA的提取,每24小时为168小时。细胞生存力进行定量通过台盼蓝染色法。整个病毒粒子中提取的后感染。提取的RNA进行定量通过qRT-PCR。

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感染,91期,
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