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Journal / Immunology and Infection / Förökning av
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Immunology and Infection

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Seven Steps to Stellate Cells
Journal (Immunology and Infection)
Seven Steps to Stellate Cells
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
Journal (Immunology and Infection)
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
 
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Förökning av

Article DOI: 10.3791/51953-v • 12:52 min • September 25th, 2014
September 25th, 2014 •
1, 1, 2, 1
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS

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Här presenterar vi ett protokoll för att propagera Homalodisca vitripennis celler och HoCV-1 in vitro. Medium avlägsnades från HoCV-1 positiva kulturer och RNA extraherades varje 24 h under 168 tim. Cellöverlevnads kvantifierades genom trypanblå färgning. Hela viruspartiklar extraherades efter infektion. Extraherad RNA kvantifierades genom QRT-PCR.

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Infektion , Cellodling Pierce sjukdom av grapevine,
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