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Journal / Neuroscience / 的vibratome切片小鼠视网膜准备感光文化…
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Neuroscience

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JoVE Journal Neuroscience
的vibratome切片小鼠视网膜准备感光文化
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的vibratome切片小鼠视网膜准备感光文化

Article DOI: 10.3791/51954-v • 11:22 min • December 22nd, 2014
December 22nd, 2014 •
1,4,5,6, 1,4,5,6, 2,4,5,6, 3,4,5,6, 4,5,6, 1,4,5,6
1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision

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对8岁天小鼠神经视网膜是在4％阿胶块的顶部。感光体层（200微米）通过的vibratome分离后，将感光体后的机械和酶解为培养接种。可用于分子，生化分析或移植的感光层。

Tags

医药，第94，初级感光细胞培养，遗传性视网膜变性，棒衍生锥活力因子，S-抗原，平板式小鼠视网膜，移植。
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