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Journal / Neuroscience / Vibratome Sectioning Mouse Retina te Fotoreceptor …
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Neuroscience

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JoVE Journal Neuroscience
Vibratome Sectioning Mouse Retina te Fotoreceptor culturen Bereid
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Vibratome Sectioning Mouse Retina te Fotoreceptor culturen Bereid

Article DOI: 10.3791/51954-v • 11:22 min • December 22nd, 2014
December 22nd, 2014 •
1,4,5,6, 1,4,5,6, 2,4,5,6, 3,4,5,6, 4,5,6, 1,4,5,6
1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision

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Neurale retina van een muis 8 dagen oud is op een 4% gelatine blok. Na isolatie van de fotoreceptor laag (200 pm) door vibratome, zijn de fotoreceptoren gezaaid na mechanische en enzymatische dissociatie voor cultuur. De fotoreceptor laag kan worden gebruikt voor moleculaire, biochemische analyses of transplantatie.

Tags

Geneeskunde Primaire fotoreceptorcel cultuur Erfelijke retinale degeneratie Rod-afgeleide Cone Leefbaarheid Factor S-antigeen Flatscreen-gemonteerde muis netvlies Transplantation.
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