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Journal / Neuroscience / Vibratome Seccionamento Rato Retina de Preparar…
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Neuroscience

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JoVE Journal Neuroscience
Vibratome Seccionamento Rato Retina de Preparar culturas Fotorreceptoras
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Vibratome Seccionamento Rato Retina de Preparar culturas Fotorreceptoras

Article DOI: 10.3791/51954-v • 11:22 min • December 22nd, 2014
December 22nd, 2014 •
1,4,5,6, 1,4,5,6, 2,4,5,6, 3,4,5,6, 4,5,6, 1,4,5,6
1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision

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Retina neural de um rato com idade entre 8 dias é em cima de um bloco de gelatina 4%. Após o isolamento da camada de fotorreceptores (200 mm) por vibratome, as células fotorreceptoras são cultivadas após dissociação enzimática e mecânica para a cultura. A camada de fotorreceptores pode ser utilizado para análises moleculares, bioquímicas ou transplante.

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Medicina Edição 94 de cultura de células fotorreceptoras Primária degeneração da retina Herdado Rod derivado Cone Viabilidade Factor S-Antigen retina Flat-montado rato Transplantation.
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