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Journal / Neuroscience / Vibratome Sektione mus Retina man förbereder…
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Neuroscience

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JoVE Journal Neuroscience
Vibratome Sektione mus Retina man förbereder ljusmätare kulturer
Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo
Journal (Neuroscience)
Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo
Author Spotlight: Unveiling Neural Mechanisms Through Automated Evaluation of Motor Learning and Myelin Plasticity Studies Using the Erasmus Ladder (Video) | JoVE
Journal (Neuroscience)
Author Spotlight: Unveiling Neural Mechanisms Through Automated Evaluation of Motor Learning and Myelin Plasticity Studies Using the Erasmus Ladder (Video) | JoVE
 
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Vibratome Sektione mus Retina man förbereder ljusmätare kulturer

Article DOI: 10.3791/51954-v • 11:22 min • December 22nd, 2014
December 22nd, 2014 •
1,4,5,6, 1,4,5,6, 2,4,5,6, 3,4,5,6, 4,5,6, 1,4,5,6
1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision

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Neurala näthinnan av en mus i åldern 8 dagar är på toppen av en 4% gelatinblocket. Efter isolering av ljusmätare lagret (200 nm) genom vibratome är de fotoreceptorer seedade efter mekanisk och enzymatisk dissociation för kultur. Den fotoreceptorskiktet kan användas för molekylära, biokemiska analyser eller transplantation.

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Medicin Primärljusmätare cellodling ärvda näthinnedegeneration Rod-härledda Cone livskraft Factor S-antigen Flat monterade mus näthinnan Transplantation.
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