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Journal / Immunology and Infection / Nucleofection İnsan THP-1 Makrofagların Yüksek…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Nucleofection İnsan THP-1 Makrofagların Yüksek Verimli Transfeksiyonu
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Nucleofection İnsan THP-1 Makrofagların Yüksek Verimli Transfeksiyonu

Article DOI: 10.3791/51960-v • 07:54 min • September 2nd, 2014
September 2nd, 2014 •
1, 1, 1
1Institute of Nutrition, Friedrich Schiller University Jena

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Bu protokol, yüksek hücre canlılığı ve farklılaşması ve polarizasyon tam makrofaj kapasitesini muhafaza ederek, yüksek transfeksiyon verimi olan elektroporasyon ile siRNA veya plazmit DNA'sı ile insan THP-1 makrofajlar transfekte etmek için etkin ve güvenilir bir yöntem sunmaktadır.

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Enfeksiyon Sayı 91 makrofajlar THP-1 transfeksiyon elektroporasyon sıRNA plazmid DNA protokol polarizasyon Nucleofection
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