剖析果蝇卵巢

Biology

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Summary

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Wong, L. C., Schedl, P. Dissection of Drosophila Ovaries. J. Vis. Exp. (1), e52, doi:10.3791/52 (2006).

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Abstract

Protocol

果蝇卵巢清扫的议定书

  1. 饲料苍蝇与酵母1-2天前,解剖他们催肥卵巢。
  2. 麻醉苍蝇使用二氧化碳流。
  3. 使用镊子,选择雌蝇。
  4. 淹没到1X PBS雌蝇。
  5. 抓斗的镊子在其较低的胸部的飞行。
  6. 拖轮在小腹与另一个镊子轻轻接触,直到在腹部的内脏器官。
  7. 寻找一双卵巢和其他器官(如肠子)脱离。
  8. 梳理除了卵巢管(如果卵巢被用于免疫或原位杂交)。
  9. 保持在冰冷的1X PBS卵巢在解剖未来飞。

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Discussion

在这里,我们展示了一个在果蝇卵巢解剖的方法。作为培养不同类型的细胞在果蝇卵巢中存在的方法尚未确定,这是一个快速的方法来获得果蝇卵巢组织。从本质上讲,我们表明,解剖果蝇卵巢涉及一个简单的两或三个步骤的过程。这些解剖卵巢的后续治疗,取决于将要执行的下游实验。

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Materials

Name Type Company Catalog Number Comments
Forceps Tool Sigma-Aldrich T43537
Glass dissecting dish Tool Pyrex 7220-85

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References

  1. Costa, A., et al. The Drosophila fragile X protein functions as a negative regulator in the orb autoregulatory pathway. Dev Cell. 8, 331-342 (2005).
  2. Tan, L., et al. An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte. Development. 128, 1159-1169 (2001).

Comments

1 Comment

  1. how to dissect ovaries from pupae?

    Reply
    Posted by: Anonymous
    January 5, 2009 - 10:23 PM

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