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High Efficiency Differensiering of Human Pluripotent stamceller til Cardiomyocytes og karakterisering med flowcytometri
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High Efficiency Differensiering of Human Pluripotent stamceller til Cardiomyocytes og karakterisering med flowcytometri

Article DOI: 10.3791/52010-v • 13:13 min • September 23rd, 2014
September 23rd, 2014 •
*1, *2, 1, 1, 3, 2, 4,5, 1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
* These authors contributed equally

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Artikkelen beskriver den detaljerte metode for å effektivt skille humane pluripotente stamceller til kardiomyocytter ved selektivt å modulere Wnt veien, etterfulgt av strømningscytometri-analyse av referansemarkører for å vurdere homogenitet og identiteten av befolkningen.

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Cellular Biology menneskeskapte pluripotent stamcelle flowcytometri regissert differensiering cardiomyocyte IRX4 TNNI3 TNNT2 MCL2v MLC2a
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