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Real Time Analisi di profilo metabolico in
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Real Time Analisi di profilo metabolico in

Article DOI: 10.3791/52026-v • 08:53 min • November 3rd, 2014
November 3rd, 2014 •
1, 1,2
1Department of Medicine, Albert Einstein College of Medicine, 2Department of Cell Biology, Albert Einstein College of Medicine

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Piccoli organoidi cripta intestinali in coltura ex vivo fornire un sistema di coltura tessuto che racchiude in sintesi la crescita delle cripte dipendenti sulle cellule staminali e la loro nicchia. Abbiamo stabilito un metodo per dosare il profilo metabolico in tempo reale in organoidi cripta del mouse primario. Abbiamo trovato organoidi mantengono le proprietà fisiologiche definite dalla loro fonte.

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Cancer Biology Numero 93 Cancro colon-rettale Topo Piccolo Intestino Cripta organoide dieta metabolismo extracellulare acidificazione Rate Tasso di consumo di ossigeno
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