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JoVE Journal Medicine
Stereologisk og flowcytometri karakterisering af leukocytsubpopulationer i modeller af Forbigående eller permanent cerebral iskæmi
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Stereologisk og flowcytometri karakterisering af leukocytsubpopulationer i modeller af Forbigående eller permanent cerebral iskæmi

Article DOI: 10.3791/52031-v • 12:42 min • December 28th, 2014
December 28th, 2014 •
*1, *1, 1, 1, 1, 1
1Unidad de Investigacón Neurovascular, Departmento de Farmacología, Falcultad de Medicina, Universidad Complutense de Madrid y Instituto de Investigación Hospital 12 de Octubre, Madrid
* These authors contributed equally

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Brain myeloide celler karakterisering efter slagtilfælde kan udføres ved stereologi anvendelse af optisk fraktioneringskolonne metode eller ved en flowcytometrisk analyse af hjernen leukocytter suspensioner. Begge er nyttige teknikker til at udføre en nøjagtig fænotypisk forskel på de vigtigste myeloide celledelmængder fundet i iskæmisk hjernen.

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Medicine hjerneiskæmi myeloide celler mellem-cerebral arterieokklusion (MCAO) stereologi optisk fraktioneringskolonne flowcytometri infiltration
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