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Journal / Neuroscience / Multi-foton Intracellulær Natrium Imaging…
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Neuroscience

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JoVE Journal Neuroscience
Multi-foton Intracellulær Natrium Imaging Kombineret med UV-medieret Focal Uncaging af glutamat i CA1 pyramideneuroner
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Multi-foton Intracellulær Natrium Imaging Kombineret med UV-medieret Focal Uncaging af glutamat i CA1 pyramideneuroner

Article DOI: 10.3791/52038-v • 10:29 min • October 8th, 2014
October 8th, 2014 •
*1, *1, 1
1Institute of Neurobiology, Heinrich Heine University Düsseldorf
* These authors contributed equally

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Vi beskriver kombinationen af ​​brændvidde UV-induceret foto-aktivering af neuro-aktive forbindelser med hel-celle patch-clamp og multi-foton billeddannelse af intracellulære natrium transienter i dendritter og pigge af hippocampusneuroner i akutte vævssnit med musen hjernen.

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Neuroscience Neurosciences to-foton mikroskopi patch-clamp UV-flash fotolyse mus hippocampus bur forbindelser glutamat hjerne skive dendritceller natrium-signaler
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