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Journal / Developmental Biology / Expression von fluoreszierenden Proteinen in
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JoVE Journal Developmental Biology
Expression von fluoreszierenden Proteinen in
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Expression von fluoreszierenden Proteinen in

Article DOI: 10.3791/52042-v • 09:31 min • January 12th, 2015
January 12th, 2015 •
1, 2, 1,3, 4, 1, 5, 2
1Département de Biologie du Développement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard

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Wir berichten hier über die robuste und effiziente Expression von fluoreszierenden Proteinen nach der mRNA-Injektion in unbefruchtete Eizellen von Branchiostoma lanceolatum. Die Entwicklung der Mikroinjektionstechnik in dieser basalen chordate wird den Weg für tiefgreifende technische Innovationen in diesem aufstrebenden Modellsystem, auch in-vivo-Bildgebung und genspezifischen Manipulationen zu ebnen.

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Entwicklungsbiologie Amphioxus Schädellose Gen-Expressionsvektoren, Mikroinjektion Protokoll Modellorganismus
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