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Immunology and Infection

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JoVE Journal Immunology and Infection
Anvendelse af Fluorescent Nanopartikler til Studieinformationen ombygning af Endo-lysosomale System af intracellulære bakterier
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Anvendelse af Fluorescent Nanopartikler til Studieinformationen ombygning af Endo-lysosomale System af intracellulære bakterier

Article DOI: 10.3791/52058-v • 11:38 min • January 2nd, 2015
January 2nd, 2015 •
1, 1, 1
1Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück

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I denne artikel beskrives metoder til syntese og fluorescerende mærkning af nanopartikler (NP'er). NPS blev anvendt i puls-chase forsøg at mærke endo-lysosomale system eukaryote celler. Manipulation af endo-lysosomale system ved aktiviteter af den intracellulære patogen Salmonella enterica blev fulgt af levende celler og kvantificeret.

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Immunology fluorescerende nanopartikler endo-lysosomal systemet mærkning intracellulære bakterier kvantitativ billedanalyse rørformede segmenter
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