Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation and Functional Analysis of Mitochondria …
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
Journal (Biology)
Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
Quantifying the Antifungal Activity of Peptides Against Candida albicans
Journal (Biology)
Quantifying the Antifungal Activity of Peptides Against Candida albicans
 

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Article DOI: 10.3791/52076-v • 09:27 min • March 23rd, 2015
March 23rd, 2015 •
1, 1, 1, 2,3,4, 1
1Chemistry Department, Elon University, 2Department of Neurobiology and Anatomy, Wake Forest School of Medicine, 3Neuroscience Graduate Program, Wake Forest School of Medicine, 4ALS Center Translational Science Unit, Wake Forest School of Medicine

Chapters

Summary
Automatic Translation

Comparison of mitochondrial membrane potential between samples yields valuable information about cellular status. Detailed steps for isolating mitochondria and assessing response to inhibitors and uncouplers using fluorescence are described. The method and utility of this protocol are illustrated by use of a cell culture and animal model of cellular stress.

Tags

Isolation Functional Analysis Mitochondria Cultured Cells Mouse Tissue Comparison Distinct Groups Healthy Disease Cellular Status Cell Metabolism Energy Production Apoptosis Evaluation Mitochondrial Perturbation Organelle-specific Function Protocol Intact Mitochondria HEK Cultured Cells Mouse Liver Spinal Cord Adaptable Other Cultured Cells Animal Tissues TMRE Mitochondrial Uncouplers Inhibitors Fluorescent Plate Reader Versatile Accessible High Throughput
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter