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Journal / Developmental Biology / Grootschalige de zebravis embryonale Hart…
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JoVE Journal Developmental Biology
Grootschalige de zebravis embryonale Hart Dissection voor Transcriptionele Analyse
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Grootschalige de zebravis embryonale Hart Dissection voor Transcriptionele Analyse

Article DOI: 10.3791/52087-v • 10:00 min • January 12th, 2015
January 12th, 2015 •
*1,2,3, *1,2, 1,2,3
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover
* These authors contributed equally

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Cardiale genexpressie profielen te analyseren tijdens zebravis ontwikkeling van het hart, heeft totaal RNA worden geëxtraheerd uit geïsoleerde harten. Hier presenteren we een protocol voor het verzamelen van functionele / kloppende harten door snelle handmatige dissectie van de zebravis embryo's tot cardiale-specifieke mRNA te verkrijgen.

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Developmental Biology zebravis embryo hart dissectie RNA RT-qPCR
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