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Journal / Developmental Biology / Storskala Sebrafisk Embryonic Hjerte Dissection…
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Developmental Biology

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JoVE Journal Developmental Biology
Storskala Sebrafisk Embryonic Hjerte Dissection for Transkripsjonell Analysis
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Storskala Sebrafisk Embryonic Hjerte Dissection for Transkripsjonell Analysis

Article DOI: 10.3791/52087-v • 10:00 min • January 12th, 2015
January 12th, 2015 •
*1,2,3, *1,2, 1,2,3
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover
* These authors contributed equally

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Å analysere hjerte genekspresjonsprofiler under sebrafisk hjerte utvikling, total RNA har å være hentet fra isolerte hjerter. Her presenterer vi en protokoll for å samle inn funksjonelle / bankende hjerter ved rask manuell disseksjon fra sebrafisk embryo for å få hjertespesifikke mRNA.

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Developmental Biology sebrafisk embryo hjerte disseksjon RNA RT-qPCR
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