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Elektrofore Mobility Shift Assay (EMSA) for studier av RNA-Protein Interaksjoner
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Elektrofore Mobility Shift Assay (EMSA) for studier av RNA-Protein Interaksjoner: The IRE / IRP Eksempel

Article DOI: 10.3791/52230-v • 12:44 min • December 3rd, 2014
December 3rd, 2014 •
1,2, 1,2, 1,2
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Department of Medicine, McGill University

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Her presenterer vi en protokoll for å analysere RNA / protein interaksjoner. Den elektroforetiske mobilitet skift analyse (EMSA) er basert på differensial migrering av RNA / protein-komplekser og fri RNA under opprinnelig gelelektroforese. Ved å bruke en radioaktivt merket RNA-probe, kan RNA / proteinkomplekser bli visualisert ved autoradiografi.

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Biokjemi RNA metabolisme mRNA oversettelse post-transcriptional genregulering mRNA stabilitet IRE IRP1 IRP2 jern metabolisme ferritin transferrin reseptor
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