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Bioengineering

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JoVE Journal Bioengineering
Prøve Forberedelse Strategier for massespektrometri Imaging af 3D cellekulturmodeller
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Prøve Forberedelse Strategier for massespektrometri Imaging af 3D cellekulturmodeller

Article DOI: 10.3791/52313-v • 08:14 min • December 5th, 2014
December 5th, 2014 •
1,2, 1,2, 1,2
1Department of Chemistry and Biochemistry, University of Notre Dame, 2Harper Cancer Research Institute, University of Notre Dame

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Immortaliserede kræft cellelinjer kan dyrkes som 3D-cellekulturer, en værdifuld model for biologisk forskning. Denne protokol beskriver massespektrometri billeddannelse af 3D cellekulturer, herunder forbedringer i prøveforberedelse platform. Målet med denne protokol er at instruere brugerne at forberede 3D cellekulturer til massespektrometri billeddannelse analyse.

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Bioengineering 3D-cellekultur massespektrometri billeddannelse cellekultur prøveforberedelse sfæroider
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