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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Article DOI: 10.3791/52350-v • 11:27 min • February 7th, 2015
February 7th, 2015 •
1, 1, 1, 1, 2, 1
1Department of Internal Medicine, Section on Gerontology and Geriatric Medicine, Wake Forest School of Medicine, 2Seahorse Biosciences

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Summary
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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.

Tags

Preparation Respirometric Assessment Mitochondria Skeletal Muscle Tissue Percutaneous Needle Biopsy Electron Transport Chain Function Human Vastus Lateralis Plate-based Respirometric Profiling Extracellular Flux (XF) Analyzer Comparison Respirometric Profiles Mitochondria Amount Respiration Measurement Consistent Results Standard Errors Western Blot Analysis Mitochondrial Marker COX IV Non-mitochondrial Tissue Marker GAPDH Non-mitochondrial Contamination Clinical Research Projects
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