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JoVE Journal Developmental Biology
Organotypische Slice Culturen voor Studies van Postnatale Neurogenese
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Organotypische Slice Culturen voor Studies van Postnatale Neurogenese

Article DOI: 10.3791/52353-v • 08:52 min • March 4th, 2015
March 4th, 2015 •
1, 2,3, 1, 1
1Department of Physiology, University of Toronto, 2Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, 3Department of Education and Research, Taipei City Hospital

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Hier beschrijven we een techniek voor het bestuderen van de hippocampus postnatale neurogenese met de organotypische slice cultuur techniek. Deze werkwijze maakt in vitro manipulatie van volwassen neurogenese en maakt de directe toepassing van farmacologische middelen aan de gekweekte hippocampus.

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Developmental Biology volwassen neurogenese Organotypische culturen hippocampus BrdU CldU immunohistochemie fluorescentie microscopie farmacologie
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