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Journal / Immunology and Infection / Modellering livssyklusen til Ebola virus Under…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Modellering livssyklusen til Ebola virus Under biosikkerhetsnivå 2 forhold med viruslignende partikler som inneholder Tetracistronic Minigenomes
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Modellering livssyklusen til Ebola virus Under biosikkerhetsnivå 2 forhold med viruslignende partikler som inneholder Tetracistronic Minigenomes

Article DOI: 10.3791/52381-v • 10:11 min • September 27th, 2014
September 27th, 2014 •
1, 1, 2, 1
1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

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Arbeid med smittsomme Ebola virus er begrenset til biosikkerhet nivå fire laboratorier. Tetracistronic minigenome holdig replikasjon og transkripsjon kompetente virus lignende partikler (trVLPs) representerer en livssyklus modellering system som tillater oss å trygt modellere flere smittsomme sykluser i henhold biosikkerhet nivå to forhold, stole utelukkende på Ebola-viruset komponenter.

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Infectious Diseases utgave 91 hemoragisk feber viral Mononegavirales Infeksjoner Ebola-virus filovirus livssyklus modellering system minigenome omvendt genetikk viruslignende partikler replikering transkripsjon spirende Morphogenesis oppføring
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