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Functionele Het oplossen en Channel activiteit Metingen van gezuiverde wildtype en mutante CFTR eiwit
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Functionele Het oplossen en Channel activiteit Metingen van gezuiverde wildtype en mutante CFTR eiwit

Article DOI: 10.3791/52427-v • 09:59 min • March 9th, 2015
March 9th, 2015 •
1, 1, 1,2,3
1Programme in Molecular Structure and Function, Hospital for Sick Children, 2Department of Biochemistry, University of Toronto, 3Department of Physiology, University of Toronto

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Hier beschreven is een snelle en efficiënte procedure voor de functionele reconstitutie van gezuiverde wildtype en mutante CFTR-eiwit dat activiteit behoudt deze chloride kanaal, die defect in Cystic Fibrosis. Jodide efflux uit opgelost proteoliposomen gemedieerd door CFTR maakt studies van het kanaal en de effecten van kleine moleculen.

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Biochemie Cystic Fibrosis CFTR zuivering reconstructie chloride kanaal kanaal functie jodide efflux versterking
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