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Developmental Biology

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Cultura e co-coltura di mouse ovaie e follicoli ovarici

Article DOI: 10.3791/52458-v • 10:41 min • March 17th, 2015
March 17th, 2015 •
1, 1, 1, 2, 1
1Centre for Integrative Physiology, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh

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Questo protocollo descrive la coltura primaria / co-coltura di topo tessuto ovarico, con ovaie di topi neonati e singoli follicoli ovarici di topi prepuberi. Le tecniche di coltura supportano sviluppo in modo altamente fisiologico, consentendo studio dell'effetto di agenti estrinseci sulla ovaio, e di interazioni tra follicoli ovarici.

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Biologia Cellulare Numero 97 biologia riproduttiva ovaio cultura tecnica follicolo ovocita cellule durale immunocitochimica
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