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RNA Isolation fra celle specielle befolkningsgrupper ved hjælp af laser-capture mikrodissektion Kombineret med Rapid immunolabeling
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RNA Isolation fra celle specielle befolkningsgrupper ved hjælp af laser-capture mikrodissektion Kombineret med Rapid immunolabeling

Article DOI: 10.3791/52510-v • 07:01 min • April 11th, 2015
April 11th, 2015 •
*1,2, *1,2, 1,2
1Department of Psychiatry and Neurosciences, Faculty of Medicine, Université Laval, 2Centre de recherche de l'Institut universitaire en santé mentale de Québec
* These authors contributed equally

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Genekspressionsanalyse af en delmængde af celler i et specifikt væv er en stor udfordring. I denne artikel beskrives, hvordan du isolere høj kvalitet total RNA fra en bestemt celle population ved at kombinere en hurtig immunolabeling metode med laser capture mikrodissektion.

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Molekylærbiologi Laser capture mikrodissektion mRNA immunolabeling genekspression dopamin neuroner RNA-sekventering QRT-PCR
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