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Immunology and Infection

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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Article DOI: 10.3791/52564-v • 10:31 min • April 8th, 2016
April 8th, 2016 •
1, 1, 2, 1, 1
1Laboratory of Medical Immunology, Department of Laboratory Medicine, Radboud University Medical Centre, 2Department of Dermatology, Radboud University Medical Centre

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Summary
Automatic Translation

We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

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Lymphocyte Isolation Human Skin Phenotypic Analysis Ex Vivo Cell Culture Skin Resident T Cells Biopsy Punch Petri Dish Mechanical Dissociation Enzymatic Dissociation Single Cell Suspension Flow Cytometry Culture Medium Immunostaining FACS Analysis Dermatology Skin Tumor Immunology Xuehui He PhD Student Sterile Tools Rinse
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