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Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits

Article DOI: 10.3791/52572-v • 10:32 min • April 15th, 2015
April 15th, 2015 •
*1, *2, 3, 4, 5, 3, 4, 1, 2, 1,4
1Department of Neurobiology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, 2Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, 3School of Electrical Engineering, Tel-Aviv University, 4School of Physics and Astronomy, Tel-Aviv University, 5Department of Informatics, Bioengineering, Robotics and System Engineering, University of Genova
* These authors contributed equally

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Summary
Automatic Translation

This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity.

Tags

Design Surface Treatment Cellular Plating Culturing Modular Neuronal Networks Functionally Interconnected Circuits Brain Functions Topological Organization In Vitro Preparations Experimental Methodology Spatially Distinct Assemblies 2D Architecture Regular Cover Slips Substrate Embedded Micro Electrode Arrays Micromachined Structures Silicon Wafer Biocompatible Polymeric Stencils Surface Coating Cellular Adhesion
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