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Bioengineering

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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Article DOI: 10.3791/52576-v • 08:35 min • February 26th, 2015
February 26th, 2015 •
*1, *1, 1, 1,2, 2
1Department for Tissue Engineering and Regenerative Medicine, University Hospital Würzburg, 2Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease, Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB
* These authors contributed equally

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Summary
Automatic Translation

The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.

Tags

Three-dimensional Skin Model Full Thickness Skin Equivalent Wound Healing In Vitro Models Human Cells Animal Models Cellular Migration Cellular Proliferation Model System Reproducible Wounds Dermal Fibroblast Collagen Type I Hydrogel Epidermal Keratinocytes Multilayered Epidermis Automated Wounding Device
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