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Probe-baseret real-time PCR metoder til kvantitativ måling af microRNA
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Probe-baseret real-time PCR metoder til kvantitativ måling af microRNA

Article DOI: 10.3791/52586-v • 10:28 min • April 14th, 2015
April 14th, 2015 •
1, 1, 1, 2, 1
1Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine, The University of Sydney, 2Biomarkers Laboratory, NHMRC Clinical Trials Centre, Faculty of Medicine, The University of Sydney

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Cirkulerende microRNA har for nylig vist sig som lovende og nye biomarkører for forskellige kræftformer og andre sygdomme. Målet med denne artikel er at diskutere tre forskellige probe-baseret real-time PCR-platforme og metoder, der er til rådighed til at kvantificere og bestemme den overflod af cirkulerende microRNA.

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Molekylærbiologi microRNA ncRNA probe assays high-throughput PCR Nanofluidics / Open Arrays reverse-transkription præ-forstærkning qPCR
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