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Isolatie van menselijke lymfatische endotheelcellen door Multi-parameter fluorescentie-geactiveerde Cell Sorting
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Isolatie van menselijke lymfatische endotheelcellen door Multi-parameter fluorescentie-geactiveerde Cell Sorting

Article DOI: 10.3791/52691-v • 07:36 min • May 1st, 2015
May 1st, 2015 •
1,2, 1,3, 1, 1,2,3, 1,2, 1,2,3
1Murdoch Childrens Research Institute, The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton

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Het doel van dit protocol is om lymfatische endotheelcellen voering de menselijke lymfatische misvorming cyste-achtige schepen en voorhuiden met fluorescentie-geactiveerde celsortering (FACS) te isoleren. Latere cel kweken en uitbreiding van deze cellen maakt een nieuw niveau van experimentele verfijning voor genetische, proteomics, functioneel en celdifferentiatie studies.

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Geneeskunde , flowcytometrische sorteren, celoppervlaktemerkers
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