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JoVE Journal
Engineering

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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
 

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Article DOI: 10.3791/52712-v 11:44 min March 20th, 2015
March 20th, 2015

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Summary

We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

Tags

Surface Enhanced Raman Spectroscopy Biomolecules EBL Fabrication Nanostructured Substrates Electron Beam Lithography Immobilization Of Biomolecules Characterization SERS Protein A Glucose Binding Protein Dopamine Binding DNA Aptamer Ligand Binding SERS Imaging Surface Nanostructure Geometry Raman Activity
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