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Journal / Developmental Biology / Isolatie en karakterisering van Satellite Cellen…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolatie en karakterisering van Satellite Cellen van Rat Head Branchiomeric Spieren
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Isolatie en karakterisering van Satellite Cellen van Rat Head Branchiomeric Spieren

Article DOI: 10.3791/52802-v • 07:37 min • July 20th, 2015
July 20th, 2015 •
1, 2, 3, 1, 1, 1
1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 2Department of Biological Structure, University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center

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Dit protocol beschrijft de isolatie van satelliet-cellen uit branchiomeric hoofd spieren van een 9 weken oude ratten. De spieren komen uit verschillende kieuwbogen. Vervolgens worden de satelliet cellen gekweekt op een plek bekleding millimeter grootte hun differentiatie te bestuderen. Deze aanpak voorkomt de uitbreiding en de passage van satelliet-cellen.

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Developmental Biology hoofd spieren levator veli palatini spier kauw- spieren kauwspieren satelliet-cellen isolatie primaire cellen gespleten gehemelte regeneratieve geneeskunde tissue engineering stamcellen differentiatie myofibers
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