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长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
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Article
DOI:
10.3791/52833-v
•
15:58 min
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July 11th, 2015
长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
July 11th, 2015
•
Mayara F. Maggioli1,2, Mitchell V. Palmer1, H. Martin Vordermeier3, Adam O. Whelan3, James M. Fosse4, Brian J. Nonnecke1, W. Ray Waters1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service, United States Department of Agriculture
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Summary
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长期培养的干扰素γ酶联免疫斑点法被用作中央记忆应答的量度并且与保护性抗分枝杆菌疫苗的反应。用该测定中,外周血单核细胞刺激与分枝杆菌抗原和IL-2 14天,使分化和扩展中央记忆T细胞。