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Journal / Immunology and Infection / Applicazione di lungo termine colta interferone-γ …
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Applicazione di lungo termine colta interferone-γ Enzyme Linked immunospot Assay per la valutazione effettori e memoria delle cellule T risposte a Bovino
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Applicazione di lungo termine colta interferone-γ Enzyme Linked immunospot Assay per la valutazione effettori e memoria delle cellule T risposte a Bovino

Article DOI: 10.3791/52833-v • 15:58 min • July 11th, 2015
July 11th, 2015 •
1,2, 1, 3, 3, 4, 1, 1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service, United States Department of Agriculture

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A lungo termine colta interferone-γ enzimatico test immunospot viene usato come misura di risposte di memoria centrale e correla con le risposte vaccino anti-micobatteri protettivi. Con questo test, le cellule mononucleari del sangue periferico sono stimolati con antigeni micobatterici e interleuchina-2 per 14 giorni, differenziazione e consentendo l'espansione delle cellule T di memoria centrale.

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Immunologia Numero 101, tubercolosi bovina le cellule T CD4 vaccino.
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