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JoVE Journal
Immunology and Infection

This content is Open Access.

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
 

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Article DOI: 10.3791/52839-v 10:27 min November 10th, 2015
November 10th, 2015

Chapters

Summary

While high resolution melting analysis offers the ability to differentiate between single nucleotide polymorphisms in a heterogeneous population, mutant allele amplification bias can increase its ability to detect alleles present at relatively low percentages within a sample. This protocol describes improvements that improve the sensitivity of high resolution melting analysis.

Tags

Sensitivity High Resolution Melting Single Nucleotide Polymorphism Genotyping Malaria Plasmodium Parasite Anti-malarial Drugs Drug Resistance Markers Parasite Populations SNP Genotyping Methods Population Surveillance HRM Analysis Fluorescent-probe Based Methods
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