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Journal / Immunology and Infection / Generation og Multi-fænotypisk High-indhold…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation og Multi-fænotypisk High-indhold Screening af
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
Journal (Immunology and Infection)
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
Journal (Immunology and Infection)
Purification of Extracellular Trypanosomes, Including African, from Blood by Anion-Exchangers (Diethylaminoethyl-cellulose Columns)
 
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Generation og Multi-fænotypisk High-indhold Screening af

Article DOI: 10.3791/52851-v • 11:44 min • May 13th, 2015
May 13th, 2015 •
1, 1, 1
1Cell Biology of Bacterial Infections, CNRS, FRE3689, CPBS, Université Montpellier

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Coxiella burnetii er en obligat intracellulær Gram-negativ bakterie er ansvarlig for zoonotisk sygdom Q-feber. Her beskriver vi fremgangsmåder til generering af Coxiella fluorescerende transposon mutanter samt automatiseret identifikation og analyse af de resulterende internalisering, replikations- og cytotoksiske fænotyper.

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Infektion Infection biologi, Vero-celler med højt indhold / high-throughput screeningsassays morfologisk analyse.
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