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JoVE Journal
Bioengineering

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Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
 

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Article DOI: 10.3791/53049-v 11:57 min April 21st, 2016
April 21st, 2016

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Summary

Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.

Tags

Protein Function Altered Protein Expression Antibody Interference Three-dimensional Reconstructions Chariot Peptide Cellular Structures Treatment Effects Nuclear Proteins RNA Interference Antibody Dosages Primary Neuronal Cultures Chariot Peptide Preparation
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