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Imaging o tráfego intracelular de APP com GFP fotoactivável
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Imaging o tráfego intracelular de APP com GFP fotoactivável

Article DOI: 10.3791/53153-v • 07:55 min • October 17th, 2015
October 17th, 2015 •
1, 1,2
1Department of Physiology and Pharmacology, Robarts Research Institute, Western University, 2Department of Clinical Neurological Sciences, Western University

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Embora o transporte de proteínas de superfície celular está relativamente facilmente estudado, visualizando o tráfico de proteínas intracelulares é muito mais difícil. Aqui, usamos construções que incorporam GFP fotoactivável e demonstrar um método para seguir com precisão a proteína precursora de amilóide do aparelho de Golgi para compartimentos a jusante e siga a sua depuração.

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Cellular Biology 104 Edição proteína precursora de amilóide foto-activável GFP de Golgi lisossomas o tráfico intracelular doença de Alzheimer β-amilóide microscopia Em células vivas
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