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Imaging de intracellulære Trafficking af APP med fotoaktiverbart GFP
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Imaging de intracellulære Trafficking af APP med fotoaktiverbart GFP

Article DOI: 10.3791/53153-v • 07:55 min • October 17th, 2015
October 17th, 2015 •
1, 1,2
1Department of Physiology and Pharmacology, Robarts Research Institute, Western University, 2Department of Clinical Neurological Sciences, Western University

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Mens transport af celleoverfladeproteiner relativt let studeres, visualisere handel med intracellulære proteiner er meget vanskeligere. Her bruger vi konstruktioner inkorporerer fotoaktiverbare GFP og demonstrere en metode til nøjagtigt at følge amyloidprecursorproteinet fra Golgi-apparatet til ned-stream rum og følge dets clearance.

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Cellular Biology amyloidprecursorprotein foto-aktiverbar GFP Golgi lysosomer intracellulær transport Alzheimers sygdom β-amyloid live-cell mikroskopi
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