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Developmental Biology

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JoVE Journal Developmental Biology
Utilizzando Analisi confocale di
Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
Journal (Developmental Biology)
Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Journal (Developmental Biology)
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
 
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Utilizzando Analisi confocale di

Article DOI: 10.3791/53162-v • 08:10 min • December 14th, 2015
December 14th, 2015 •
1, 2, 3, 4, 5, 5
1Department of Biomedical Science, The Bateson Centre, University of Sheffield, 2Institute of Genetic Medicine, Newcastle University, 3Department of Cardiovascular Science, The Bateson Centre, University of Sheffield, 4School of Biochemistry, University of Bristol, 5Biology Department, University of York

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Il manoscritto qui fornisce un semplice insieme di metodi per analizzare la secrezione e la diffusione di fluorescenza ligandi marcati nel Xenopus. Ciò fornisce un contesto per valutare la capacità di altre proteine ​​di modificare la distribuzione ligando e permettendo esperimenti che possono dare comprensione dei meccanismi che regolano gradienti morphogen.

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Biologia dello Sviluppo Numero 106 Riccio senza ali GFP RFP biologia dello sviluppo patterning leganti secreti segnalazione cellulare
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