JoVE Journal
Biology
Biology
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Summary
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本文介绍了详细的协议和必要的跨膜电位(V M)双光学测绘和免费内部肌浆网(SR)设备钙在的Langendorff灌注兔心脏。此方法允许直接观察和第V 米量化和SR 的 Ca 2+动力学在完整心脏。